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1- CCD Detector:
A wide range of application
specific CCD cameras can be used
with the confocal device including:
Cooke (Sensicam QE, Sensicam EM),
Roper (CoolSNAP™ ES, CoolSNAP™ HQ,
Cascade™), Hamamatsu® (ORCA ER, AG),
and more.
2 - Light Source:
A long life Hg/metal halide arc source, coupled to the instrument
via an alignment-free light guide, allows for full spectrum
(360 nm – 700 nm) confocal imaging of virtually any fluorescent
probe.
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3- Touch Pad Control:
Control of and communication with the CARV II is accomplished
via the RS-232 serial port at the back of the touch screen assembly.
All automation can be controlled via the touch pad or it can be
connected to a PC host that is running a standard terminal
program or an application designed to integrate the CARV II into
an imaging system.
4 - Easily Switch Between Confocal and Widefield
Imaging/Viewing Modes:
The CARV II Confocal Imager permits direct viewing of confocal
images through a binocular eyepiece and/or through the camera
for fast imaging setup. It is the only pinhole spinning disk
fluorescence confocal systems which allows the user to quickly
switch from confocal to wide-field viewing or recording.
5 - Automated Filter Changers:
Automation of internal multi-position
excitation (8 position), dichroic (5 position)
and emission (8 position) filter wheels
allows fast multi-dimensional confocal
imaging.
The automated filters reduce the reliance
on multi-band pass filter sets allowing
maximum light throughput and fast
sequential imaging of up to five or more
fluorescent probes in the same sample.
6 - FRAP Capabilities:
A FRAP slit at the same focal plane as the confocal disk creates an
adjustable rectangular aperture on the image.
Passing high-intensity Hg/metal halide light through this slit
enables controlled photobleaching of part of the sample
followed by fluorescence recovery recording.
7- Microscope Compatibility:
The CARV II Confocal Imager unit can be configured
to most inverted and upright fluorescent microscopes
converting your existing fluorescence microscope
to a confocal imager.
8 - Imaging Software:
A range of state of the art 3D software packages
can be used for acquisition and analysis of
confocal images including IPLab (Scanalytics)
and Metamorph (Molecular Devices).
Command sets needed to control the CARV II are
available on request.
