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Download our brochureBrochure CrestOptics 2017.pdf
 
 
 
Fluorescence recovery after photobleaching (FRAP) on Hela Cells   Fluorescence recovery after photobleaching (FRAP) on Hela Cells

This video shows a fluorescence recovery after photobleaching (FRAP) experiment performed using Crest X-Light Confocal FRAP unit on Hela Cells expressing an ER membrane-targeted GFP, Olympus BX51WI, objective 40x water immersion – Courtesy of Crisel-Instruments S.p.A. - Rome - ITALY.
In this technique, a small region of interest is selectively hotobleached with a high intensity laser. Analysis of the luorescence recovery can be used to determine kinetic parameters of tagged proteins or other molecules, including their diffusion coefficients, pool fraction, transport rates, or binding/dissociation from other molecules.

 
Mouse Kidney Axial Sectioning   Mouse Kidney Axial Sectioning

Three channels (Dapi, Alexa Fluor 488 WGA, Alexa Fluor 568 Phalloidin,) axial stack of Mouse Kidney sample. The video shows a comparison (not the same field of view) between Wide field, Confocal Spinning Disk and VCS super-resolution acquisitions of 8 microns kidney section. The total thickness of the sample is approximately 16 microns.
The VCS reconstruction shows a much more clear axial localization of the nuclei (DAPI) within other cell structures with respect to the other techniques.

 
Improved axial resolution for amazing 3D reconstructions   Improved axial resolution for amazing 3D reconstructions

This video shows, using three different microscopy acquisition modes offered by CrestOptics confocal and super-resolution system, the improvement of 3D reconstruction of 1μm extended objects. The acquisition modes are: widefield microscopy, confocal spinning disk microscopy and VCS super-resolution microscopy.
In widefield microscopy, 1μm microspheres are at the axial resolution limit for the objective used (oil 60x 1.4 NA).
Being able to resolve objects does not always imply being able to get good quality images and high deblurring levels in the final reconstructions. What is shown here, indeed, is a further improvement when going from confocal spinning disk mode to VCS mode.

 

 
Fuorescent Confocal Microscopy   CrestOptics -- Fluorescent confocal microscopy with X-Light Confocal Imager System 1

Color combined time lapse images at a single confocal plane of a sand dollar embryo during mid blastula stage (rhodamine-tubulin and Alexa 488 phalloidin, Molecular Probes, Eugene, Oregon).

 
Confocal microscopy   CrestOptics -- Fluorescent confocal microscopy with X-Light Confocal Imager System 2

Confocal 3D reconstruction of GFP tagged membrane receptor in Agrobacterium tumefaciens leaves with CARV LX spinning disk system by Crest.

Courtesy of Prof. Giulia De Lorenzo, Università "La Sapienza" di Rome, Italy.

 
Confocal microscopy CrestOptics -- Fluorescent confocal microscopy with X-Light Confocal Imager System 3

Maximum projection and orthogonal view created from confocal sections through a sea urchin embryo fixed and stained with anti-tubulin (green) and phalloidin.

 
Stack Montage Overlay   Stack Montage Overlay

Maximum projection and orthogonal view created from confocal sections through a sea urchin embryo fixed and stained with anti-tubulin (green) and phalloidin.

 

Crestoptics S.p.A.

Via Mattia Battistini, 184/D - 00167 ROMA

Tel. 0039.06.61660508 - FAX 0039.06.61662738

e-mail: info@crestopt.com

 

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